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Biochemical and Immunochemical Techniques – A Laboratory Manual


Dr. Ashok Kumar Krishna Kumar

Associate Professor & Head i/c in the Department of Biotechnology, School of Life Sciences, Vels Institute of Science Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamil Nadu, India.

Dr. M. Jayanthi

Assistant Professor in the Department of Biotechnology, School of Life Science, Vels Institute of Science Technology and Advanced Studies (VISTAS), Pallavaram, Chennai, Tamil Nadu, India .

ISBN: 978-93-94174-94-8

Publisher: Excellent Publishers

Language: English

Publication Year: 2024

Binding: Paperback

Table of contents
Front Matter
Pages i-xIII



Welcome to the Biochemical and Immunochemical Manual, a comprehensive guide designed to aid researchers, students, and professionals in the fields of biochemistry and immunology. This manual has been meticulously curated to provide a diverse array of protocols, techniques, and methodologies essential for conducting experiments, analysing data, and interpreting results in these dynamic disciplines. Biochemistry and immunology are fundamental branches of science that intersect and intertwine in myriad ways. Biochemical processes underpin the functioning of cells, organisms, and biological systems, while immunology delves into the complex mechanisms by which organisms defend themselves against pathogens and maintain immune homeostasis. The convergence of these fields is critical for understanding the molecular basis of health and disease, and for advancing medical research and therapeutic interventions. In this manual, you will find a wealth of information ranging from basic laboratory techniques to advanced experimental procedures. Each experiment is meticulously crafted by experts in the respective fields, providing clear, concise, and practical guidance. The Biochemical Techniques section covers the estimation and detection of different biochemical substances such as Glucose, Carbohydrates, Proteins, Urea and Uric acid. The Immunochemical part covers the Antigen/Antibody detection and Interaction. The interaction of Ag/Ab helps in the detection of the complex formed. We recognize that scientific research is an ever-evolving endeavour, marked by continual innovation and discovery. Therefore, this manual is intended to be a dynamic resource, adaptable to the evolving needs and advancements in biochemistry and immunology. As such, we encourage feedback, suggestions, and contributions from our readers to ensure that future editions remain current, relevant, and comprehensive. It is our sincere hope that this manual will serve as a valuable resource for students, educators, researchers, and practitioners alike, fostering a deeper understanding and appreciation for the intricate workings of biochemistry and immunology. May it inspire curiosity, spark creativity, and facilitate groundbreaking discoveries in the pursuit of scientific knowledge and medical progress.

The content of the book is as follows:

    Biochemical Techniques
  • Experiment -I Basic Calculation For Reagent Preparation
  • Experiment -II Preparation of Buffer Solution and Measurement of pH
  • Experiment -III Qualitative Analysis of Carbohydrate
  • Experiment -IV Qualitative Analysis of Amino Acids
  • Experiment -V Qualitative Analysis of Lipids
  • Experiment -VI Quantitative Analysis of Carbohydrates by Anthrone Method
  • Experiment -VII Quantitative Analysis of Protein by Lowrys Method
  • Experiment -VIII Quantitative Analysis of Reducing Sugar by DNSA Method
  • Experiment -IX Test For Fatty Acid (Saponification)
  • Experiment -X Collection, Preservation & Qualitative Analysis of Urine Sample
  • Experiment -XI Estimation of Urea by Diacetyl Mono-Oxime Thiosemicarbazide Method
  • Experiment -XII Estimation of Uric Acid
  • Experiment -XIII Estimation of Serum Protein by Biuret Method
  • Immunochemical Techniques
  • Experiment -I Anti Streptolysin O (ASO) Test
  • Experiment -II Radial Immunodiffusion
  • Experiment -III Double Immuno-Diffusion
  • Experiment -IV Immunoelectrophoresis
  • Experiment -V Rapid Plasma Reagin (RPR) Test
  • Experiment -VI Coomb’s Test
  • Experiment -VII Venereal Disease Research Laboratory (VDRL) Test
  • Experiment -VIII Widal Test
  • Experiment -IX Latex Agglutination Test
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